Table 2.

Influence of 30-min or 60-min equilibration in 0 (control), 0.5, 2.0, or 5.0 mM of AICAR on sperm motion (SCA program analysis) in fresh or frozen-thawed epididymal cat spermatozoa. Values are expressed as mean ± SD (n = 10 cats)

Metrics		Fresh	Control	0.5 mM AICAR	2.0 mM AICAR	5.0 mM AICAR
VCL (μm/s)	Cooled sperm	69.3 ± 5.5 A
	30 min		68.1 ± 13.3A,a	70.9 ± 10.7A,a	66.5 ± 9.4A,a	57.3 ± 17.1B,a
	60 min		60.3 ± 2.9B,b	71.5 ± 2.1A,a	58.1 ± 0.7B,b	49.5 ± 2.3C,b
	Cryopreserved sperm
			58.2 ± 2.2A,a	65.7 ± 4.4B,a	61.3 ± 9.1A,B,a	48.7 ± 11.2B,a
	30 min		57.4 ± 4.9A,a	64.3 ± 5.1B,a	54.4 ± 8.3A,a	44.1 ± 6.6C,a
	60 min
VAP (μm/s)	Cooled sperm	36.1 ± 5.1A
	30 min		39.1 ± 6.5A,a	40.2 ± 6.1A,a	34.3 ± 3.4A,a	32.5 ± 6.7A,a
	60 min		35.9 ± 1.1A,a	34.8 ± 2.8A,a	33.9 ± 1.6A,a	25.2 ± 4.5B,b
	Cryopreserved sperm
			30.7 ± 7.8A,B,a	32.9 ± 6.2A,a	29.6 ± 4.1A,B,a	27.5 ± 3.8B,a
	30 min		31.1 ± 5.8A,a	29.2 ± 4.5A,a	28.4 ± 6.7A,a	22.6 ± 5.9B,a
	60 min
VSL (μm/s)	Cooled sperm	48.3 ± 2.6 A
	30 min		46.3 ± 3.7A,a	47.3 ± 4.0A,a	44.8 ± 2.6A,a	41.2 ± 1.1B,a
	60 min		44.9 ± 3.4A,a	48.1 ± 2.2A,a	38.8 ± 3.1B,b	35.4 ± 3.7B,b
	Cryopreserved sperm
	30 min		38.2 ± 2.2A,a	43.9 ± 2.1B,a	39.3 ± 3.6A,B,a	34.8 ± 5.7B,a
	60 min		39.1 ± 3.8A,a	42.9 ± 3.4A,a	34.7 ± 4.4B,a	30.8 ± 6.9B,a
ALH (μm)	Cooled sperm	2.3 ± 0.9 A
	30 min		2.5 ± 0.7A,a	2.7 ± 0.7A,a	2.8 ± 0.6A,a	3.1 ± 0.7B,a
	60 min		3.3 ± 0.4B,a	3.3 ± 0.7B,a	2.9 ± 0.5A,a	3.3 ± 0.5B,a
	Cryopreserved sperm
			2.6 ± 0.3A,a	2.7 ± 0.9A,a	3.1 ± 0.7A,a	3.3 ± 0.4A,a
	30 min		3.5 ± 0.6A,b	3.0 ± 0.8A,a	3.0 ± 0.3A,a	3.7 ± 0.6A,a
	60 min

Fresh sample data were only compared with cooled samples

Within the same row, values with different uppercase letters between treatment groups are statistically different (P ≤ 0.05)

Within a treatment group, values with lowercase letters between incubation times are statistically different (P ≤ 0.05)