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. 2019 Jun 24;45:181–191. doi: 10.1016/j.ebiom.2019.06.023

Fig. 1.

Fig. 1

Expression and prognostic significance of TCF7 in PHCC. (A) Heatmap and hierarchical clustering analysis revealed the different Wnt-related gene expression profiles in three pairs of PHCC tissues (T1, T2, and T3) and adjacent normal tissues (N1, N2, and N3). High and low expression levels are indicated in red and blue, respectively. (B) mRNA levels of TCF family were determined by mRNA sequencing in FPKM method. (C) qRT-PCR was performed to detect TCF7 and LEF1 mRNA levels in PHCC tissues and adjacent normal tissues. (D) Western blotting was performed to compare TCF7 expression in PHCC tissues and adjacent normal tissues. (E) Representative images of immunohistochemical staining for TCF7 in PHCC and normal bile duct specimens in the tissue microarray (top, 100× magnification; bottom, 400× magnification). (F) IHC scores for TCF7 in 39 pairs of PHCCs and adjacent normal tissues. (G and H) Overall survival curves for patients with PHCC in cohort 1 (G) and cohort 2 (H) were stratified according to TCF7 expression (Kaplan-Meier method). Patients with high TCF7 expression had significantly poorer overall survival rates than those with low TCF7 expression. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)