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. 2019 Jul 18;21(9):849–862. doi: 10.1016/j.neo.2019.06.004

Figure 3.

Figure 3

miR-125a-5p regulates cell migratory and invasive ability in vitro. Transfection of miR-125a-5p into HN5 (A) and UM-SCC-22B (B) cell lines results in decreased wound healing, compared to control miRNA, in an in vitro wound healing assay. Cells were fixed in glutaraldehyde and stained with crystal violet. Brightfield microscopy images were taken with a 10x objective. Cell invasion was assessed with a Matrigel invasive chamber. Cells were transfected with miRNA mimics and then placed into the chamber for a period of 24 hours. The cells that were embedded in the Matrigel were stained using crystal violet and counted using brightfield microscopy with a 10x objective. There were decreased cells invading into the Matrigel with transfection of miR-125a-5p mimic, compared to the control miRNA, in both HN5 (C) and UM-SCC-22B (D) cells. Data were analyzed with the Student's t-test and are presented as the mean ± standard deviation. The in vitro scratch assay was carried out in triplicate. Five 10x fields were analyzed for the in vitro invasion assay.