Fig. 4.

Atp6v1b2 c.1516C > T causes behavioral deficits in mice. (a) NOR test.Left: In a short period, (Day 2), the time spent on exploring the new block was significantly longer than that spent on exploring the old block for WT group (**P < 0.01, Student's t-test). However, the Atp6v1b2^Arg506X/+ and Atp6v1b2^{Arg506X/Arg506X} groups showed no difference in exploration time between the new and old blocks (P > 0.05, Student's t-test, WT, n = 10; Arg506X/+, n = 11; Arg506X/Arg506X, n = 9). Right: In a long period (Day 3), the time spent on exploring old or new blocks showed no difference (P > 0.05, Student's t-test) for either WT, Atp6v1b2^Arg506X/+, or Atp6v1b2^{Arg506X/Arg506X} mice. (b) PAT was used to examine learning and memory abilities. WT mice had a shorter latency time on day 2 compared to Day 1 (*P < 0.05, Student's t-test). After foot shock on Day 2, the latency time on Day 3 was longer than that on Day 2 (**P < 0.01, Student's t-test) and day 1 (*P < 0.05, Student's t-test). However, the latency time of Atp6v1b2^{Arg506X/Arg506X} and Atp6v1b2^Arg506X/+ mice showed no significant difference among the three days (P > 0.05, Student's t-test). In addition, WT mice had a longer latency time on Day 3 than Atp6v1b2^{Arg506X/Arg506X} mice (*P < 0.05, Student's t-test). (c) Atp6v1b2^{Arg506X/Arg506X} mice had epilepsy in the MWM test.