Fig. 5.

Adoptive transfer of B cells drives the bm12 inducible lupus-like response in SMS1 deficiency mice.

The WT and SMS1 knockout mice were transferred i.v. with 10 million purified B cells from C57BL/6 mice and control group was injected with 300 μL PBS. Six hours after transfer, the three groups were induced for cGVHD lupus-like model. (a) Serum ANA titers, and (b) the urine protein/Cr were detected at the 4 weeks after induction. (c-d) Weight and lymphocytes number of spleens were measured. (e-g) The percentage and MFI of CD69, CD80 and CD86 of B-lymphocytes were assessed by flow cytometry, respectively. Data presented as mean ± SEM and p-values are calculated using unpaired student's t-tests, two-tailed. ^⁎p < .05, ^⁎⁎p < .01, ^⁎⁎⁎p < .001.