Supplementary Fig. 2.

FACS gating for Immune cell profiling of the spleen. Representative gating of flow cytometry performed at days 0 (control) and 14 (2% DSS and 2% DSS + TUS) in the spleen for CD4⁺, CD8⁺, CD25⁺, F4/80⁺ and B220⁺ cells. F4/80⁺ macrophages and CD3⁺CD4⁺CD25⁺ T cells were enriched through magnetic separation before flow cytometry analysis.