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. 2019 Jul 2;45:563–577. doi: 10.1016/j.ebiom.2019.06.039

Fig. 6.

Fig. 6

VD3-VDR axis modulates gene expression in AM following LPS stimulation. a-b, Genome-wide transcriptional analysis of AM stimulated with LPS or LPS plus VD3 for 4 h. Heatmap (a) and overlap (b) of LPS-induced genes and VD3-induced genes in the presence LPS. LPS-induced genes were identified by comparison of LPS stimulation to control. VD3-induced genes were identified by comparison of LPS plus VD3 to LPS. c, Comparison of Tnfa, Il1b, Cxcl2, Ccl5, Ccl2, Pdgfb and Irf4 transcript levels by qPCR in AM without any stimulation, with LPS or LPS plus VD3 stimulation for 4 h. d. Flow cytometry analysis of Irf4 intracellular staining in wildtype (WT) and Vdr−/− AM with or without VD3 treatment for 24 h. e, Genome-browse of VDR-binding peaks in Irf4 by IGV. f, Comparison of ChIP-seq peak scores of VDR-binding genes and LPS-induced genes (>2-fold changes) in AM. g, Genome-browse of VDR-binding peaks in Il1b and Ccl2 by IGV.