NDP52 Interacts with ULK1 Complex through FIP200
(A and B) Western analysis of immunoprecipitation assay of endogenous FIP200 (B) or ULK1 (A) in HeLa cells.
(C) Endogenous immunoprecipitation of NDP52 from WT HeLa cells treated with oligomycin/antimycin (OA) and Bafilomycin (200 nM; to prevent receptor degradation) for 3 h. Cells were crosslinked in situ using DSP prior to immunoprecipitation and western blot analysis.
(D) Western analysis of immunoprecipitation assay for NDP52 in either WT or FIP200 KO HeLa cells.
See also Figure S1.