Fig. 1.

PLAGL2 and POFUT1 are positively correlated in human tumors.

(a) The TCGA dataset showed that PLAGL2 was co-expressed with POFUT1 in 32types of human cancer tissues; the Pearson correlation coefficient in colorectal tissues ranked first and second. The abbreviated names of all cancers are listed here; full names and co-expression analysis can be found in Supplemental Table 4 or TCGA website (https://cancergenome.nih.gov/) and ChIPBase v2.0 (http://rna.sysu.edu.cn/chipbase/).

(b) PLAGL2 co-expressed with POFUT1 in 292 colorectal tumor tissues (GEO: GSE14333) and (c) 65 rectal tumor tissues (GEO: GSE 20842) and (d) 111 colorectal tumor tissues (GEO: GSE20916).

(e) Correlation of PLAGL2 and POFUT1 mRNA levels analyzed by RT-PCR in 21 colorectal cancer tissues.

(f) Correlation of PLAGL2 and POFUT1 mRNA levels analyzed by RT-PCR in 5 colorectal cancer cells.

(g) Seven pairs of colorectal carcinoma tissues and adjacent normal tissues were randomly selected for Western blot analyses. (odd numbers represent paired colorectal cancer tissues and even numbers represent paired adjacent tissues of colorectal cancer)

(h) Correlation of PLAGL2 and POFUT1 protein levels analyzed by Western blot in 7 pairs of colorectal carcinoma tissues and adjacent normal tissues.