Fig. 3.

PLAGL2 and POFUT1 maintenance of co-expression may not be through the 3′-untranslated regions of both genes.

(a) Schematic outlining the two fragments PLAGL2 3’ UTR1 and 3’UTR2 used for overexpression and luciferase experiments.

(b) qRT-PCR of lentivirus-mediated PLAGL2 3’UTR overexpression, and the POFUT1 and PLAGL2 expression in response to overexpression of PLAGL2 3’UTR in HCT-116 and SW620 cells.

(c) WB showing POFUT1 protein in response to overexpression of PLAGL2 3’UTRs in SW620 and HCT-116 cells.

(d) Quantification of (C).

(e) Luciferase activity in SW620 cells cotransfected with luciferase-PLAGL2 3’UTR2 reporter construct and ShRNA against POFUT1.

(f) qRT-PCR in lentivirus-mediated PLAGL2 and POFUT1 overexpression and cross regulation in HT-29 and HCT-116 cells.

(g) Western blot in lentivirus-mediated PLAGL2 and POFUT1 overexpression and cross regulation in HT-29 and HCT-116 cells. EV: empty vector.