Fig. 7.

Mutations in shared transcription factor binding sites in the PLAGL2/POFUT1 bidirectional promoter region lead to decreased promoter activity in both directions.

(a) Schematic outlining the 6 mutations in the 179 bp promoter sequence (more details can be found in Supplementary Table 2).

(a) Schematic outlining the 6 mutations in the 179 bp promoter sequence (more details can be found in Supplementary Table 2).

(b) Activity of mutated and wild-type forms of the PLAGL2/POFUT1 promoter region in HCT116 cells.

(c) Fluorescence microscopy analysis of simultaneous expression of the fluorescent proteins mCherry (red) and EGFP (green) driven by the 179 bp POFUT1/PLAGL2 promoter region with or without mut1 mutation in HCT116 cells. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)