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. 2019 Jul 4;45:124–138. doi: 10.1016/j.ebiom.2019.06.051

Supplemental Fig. 2.

Supplemental Fig. 2

Combined PLAGL2 /POFUT1 inhibition have greater effects on opposing tumor. (a and b) HCT116 and SW480 were stably transduced with lentiviral vectors carrying PLAGL2 or /and POFUT1-specific small hairpin RNAs. The control group contained lentiviral vectors carrying negative control shRNA (shNC). Silencing of both or single genes was confirmed by RT-PCR (a) and Western blot analysis (b). (c) The EdU proliferation assay was performed after shRNA-mediated suppression of POFUT1 or/and PLAGL2 in SW480 cells. Violin plots in the right panel present quantification. Shown here are the results for the ShPLAGL2–2 and ShPOFUT1–2 sequences (scale bar:50 μM). (d) Suppressing PLAGL2 or/and POFUT1 expression reduces anchorage-independent growth in HCT116 cells. Violin plots in the right panel present quantification. Shown here are the results for the ShPLAGL2–2 and ShPOFUT1–2 sequences (scale bar:100 μm); d: day. (e) Representative images of invasion assays showing HCT116 and SW480 cells stably transduced with lentiviral vectors carrying PLAGL2 or /and POFUT1-specific small hairpin RNAs after 36–72 h induction with 10% FBS followed by crystal violet (0.1%) staining. Violin plots in the right panel presented quantification. Shown here are the results for the ShPLAGL2–2 and ShPOFUT1–2 sequences.