Fig. 1.

Insulin-responsive temporally differentially expressed genes. (A) RNA-seq of 10 different time points at 20-min intervals (3 biological replicates per time point) after insulin stimulation of Drosophila S2R+ cells. (B) RNA-seq data for Thor and Myc, both known to be transcriptionally regulated by insulin signaling in Drosophila. (C) 163 genes overlapped between 1,254 insulin-regulated genes and 750 previously identified nucleolus regulators. (D) Scheme for identification of genes affecting nucleolar morphology/growth. (E) Control ventrolateral muscles from third instar larvae stained for DAPI (nucleus) in blue and anti-Fibrillarin (nucleolus) in red.