Fig. 2.

Identification of Myc-dependent nucleolus regulators in Drosophila. (A) List of 33 genes that were lethal when knocked down and were required for normal nucleolus morphology. (B and C) Area of α-Fibrillarin (nucleolus) stain following knockdown of each gene by Dmef2-Gal4; UAS-RNAi (B) or simultaneous knockdown of Myc and the 10 genes with the highest-scoring nucleolar area phenotype (C). Circles represent the total areas from single VL4 muscles from individual larvae. Horizontal lines denote the grand mean. (D) Dmef2-Gal4 knockdown of Surf6 causes enlarged nucleolus and nucleus compared with control white knockdown. Concurrent knockdown of Myc blocks the Surf6 nucleolus phenotype. DAPI (nucleus) is in blue, and α-Fibrillarin (nucleolus) is in red.