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. 2019 Jul 2;116(29):14708–14713. doi: 10.1073/pnas.1902250116

Fig. 2.

Fig. 2.

Monoallelic coactivation of Iγ3 and Iγ2b promoters upon LPS stimulation. (A) Scheme representing the two alleles of ZI/WT mice. On the ZI allele, a constitutive insulator (cHS4) has been inserted upstream of 3′RR, thus blocking activation of Iγ3 and Iγ2b promoters. Switch transcription can occur only on the WT allele. (B) Percentages of single- and double-expressers upon single-chromosome RT-qPCR. Purified B cells from n = 2–3 ZI/WT mice were grown in the absence (0 h) or presence of LPS for 12–42 h. The percentages of Sµ+ (orange), Sγ3+ (blue), Sγ2b+ (yellow), and Sγ2b+Sγ3+ (green) change with time (n indicates the number of single cells analyzed per time point). (C) Evolution of the total number of single- versus double-expressers with time after stimulation (same data as for B). (D) Evolution of the total number of Sγ3+ or Sγ2b+ alleles (single- plus double-expressers) with time after stimulation (same data as for B). (E) Graphic representation of the percentage of single Sγ3 (blue) or single Sγ2b (yellow) expressers versus double-expressers (green) contributing to the total number of Sγ3+ or Sγ2b+ alleles with time post stimulation (same data as for B).