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. 2019 Jul 2;116(29):14708–14713. doi: 10.1073/pnas.1902250116

Fig. 3.

Fig. 3.

Premature activation of Iγ3 does not preclude activation of Iγ2b. (A) Scheme representing the two alleles of ZI/5′hs1RIΔ mice. While in the ZI allele the 3′RR is unable to activate the Iγ3 and Iγ2b promoters after LPS stimulation, in the 5′hs1RIΔ allele, the two promoters are already active before induction due to deletion of the 5′hs1RI insulator. (B) Percentages of single- and double-expressers upon single-chromosome RT-qPCR. Purified B cells from n = 2–3 ZI/5′hs1RIΔ mice were grown in the absence (0 h) or presence of LPS for 12–42 h. The percentages of Sµ+ (orange), Sγ3+ (blue), Sγ2b+ (yellow), and Sγ2b+Sγ3+ (green) change with time (n indicates the number of single cells analyzed per time point). (C) Evolution of the total number of single- versus double-expressers with time after stimulation (same data as for B). (D) Graphic representation of the percentage of single Sγ3 (blue) or single Sγ2b (yellow) expressers versus double-expressers (green) contributing to the total number of Sγ2b+ or Sγ3+ alleles with time post stimulation (same data as for B). (E) Comparison of the percentage of single Sγ3 expressers with time post stimulation in ZI/WT and ZI/5′hs1RIΔ mice (same data as for B and Fig. 2B).