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. 2019 Jul 2;116(29):14708–14713. doi: 10.1073/pnas.1902250116

Fig. 4.

Fig. 4.

Promoters that respond to stimuli other than LPS compete for the 3′RR activity. (A) Scheme representing the two WT alleles from 129Sv1 and C57BL6J strains. Iγ1 and Iε promoters are induced by IL4. The Sγ1 and Sε transcripts originating from the two alleles can be distinguished by using polymorphic primers. (B) Percentages of single- and double-expressers upon single-chromosome RT-qPCR. Purified B cells from n = 2 129Sv1/C57BL6J mice were grown in the absence (0 h) or presence of IL4 for 42 h. The percentages of Sµ+ (orange), Sγ1 (red), Sε (green), or both Sγ1 and Sε (purple) are indicated (n indicates the number of single cells analyzed). (C) Graphic representation of the percentage of Sγ1 single-expressers (red) or Sε single-expressers (green) versus double-expressers (purple) contributing to the total number of Sγ1+ and Sε+ cells in each allele (same data as in B). (D) Iγ2b and Iα promoters are induced by TGF-β. (E) Percentages of single- and double-expressers upon single-chromosome RT-qPCR. Purified B cells from n = 3 129Sv1/C57BL6J mice were grown in the absence (0 h) or presence of TGF-β for 42 h. The percentages of Sµ+ (orange), Sγ2b+ (yellow), Sα+ (gray), or Sγ2b+Sα+ (green) are indicated (n indicates the number of single cells analyzed). (F) Graphic representation of the percentage of Sγ2b single-expressers (yellow) or Sα single-expressers (gray) versus double-expressers (green) contributing to the total number of Sγ2b+ and Sα+ cells in each allele (same data as in E). (G) Comparison of the percentage of Sγ2b double-expressers after LPS (Sγ3+Sγ2b+) or TGF-β (Sγ2b+Sα+) stimulation (same data as for B and Fig. 2B).