Fig. 3.

Testing the accuracy of NuRIM. (A) Haploid nuclei are smaller than diploid nuclei, but the recovered Nup positions in both cases were highly consistent (pairwise rmsd = 0.7 nm; error bars always represent the SEM except when stated). Schematics illustrates the general notion of the pairwise rmsd serving to assess the accuracy of structural methods. (B) Another strategy to test NuRIM accuracy was to compare the predicted average positions for double mutants with the positions actually measured (pairwise rmsd = 2.7 nm). (C) To test NuRIM’s robustness against variations in background fluorescence, soluble yEGFP-NES and yEGFP-NLS were overexpressed on top of Nup84-yEGFP (pairwise rmsd = 2.4 nm) (36). (Scale bars, 1 μm.)