Fig. 5.

Characterizing FG repeats using NuRIM. (A) Comparing results from C-terminal versus N-terminal tagging of Nup116 revealed that the flexible region of Nup116 (including both its FG-repeat region and a short linear interaction motif [SLIM]) are directed on average toward the core scaffold Nup188 (Δh = 8.2 ± 3.1 nm). INM and ONM indicate the inner and outer nuclear membranes, respectively. (B) Repressing the scaffold Nup188 led to a partial release of Nup116 toward the cytoplasm (Δh = 3.8 ± 0.5 nm) (see also figure 4G in ref. 20). (C) Comparing the positions of C-terminally versus N-terminally tagged Nup159 confirms the view that cytoplasmic-facing FG Nups more typically protrude into the cytoplasm (Δh = 3.1 ± 2.2 nm).