Table 1.

Accuracy of NuRIM evaluated using various approaches

Approach	Rmsd accuracy, nm	Comments
Nup positions in haploid strains compared with diploid strains (Fig. 3A)	0.7	4 haploid Nup-yEGFP strains versus matching diploids (Dataset S1, sheet 2)
Positions in double mutants compared with predicted values based on data from single mutants (Fig. 3B)	2.7	5 double-mutant combinations (Dataset S1, sheet 3)
Positions in base strain compared with strains where soluble yEGFP is enriched in the cytoplasm or nucleoplasm (Fig. 3C)	2.4	2 Nup-yEGFP base strains, 6 Nup-yEGFP strains in total (Dataset S1, sheet 4)
Positions in base strain compared with strains with 102-aa linkers to yEGFP	1.6	3 Nup-yEGFP base strains (Dataset S1, sheet 5)
Positions in base strain compared with superfolder GFP strains	1.1	4 Nup-yEGFP base strains (Dataset S1, sheet 6)
Positions in Y complex obtained using 2 different NE reference probes (Fig. 4C)	1.9	5 Nup-yEGFP strains from the Y complex (Dataset S1, sheet 7)
Average accuracy	1.55