Fig. 2.

SO1989 improves the white adipose inflammation. (a–b) The concentration of TNFα and MCP-1 in serum of HFD mice treated with vehicle, indicated doses of CDDO-Me or SO1989 for 15 days were quantified by ELISA assay according to manufacturer's instructions (n = 5–6). (c–d) Representative H&E staining showed adipose tissue morphology of the HFD-fed mice treated with the vehicle (0.1% DMSO), indicated doses of CDDO-Me or SO1989 for 15 days, original magnification ×200, (n = 5–6). Arrows indicated the inflammatory cells in adipose tissue. The Quantification of inflammatory cells infiltration for five sections/200× field, five to six fields/gland/mouse. (e–f) epididymal adipose tissues were homogenized and the total RNA was isolated by using Trizol reagent, followed by qRT-PCR with the indicated probes (n = 5). The 36B4 was used as an endogenous control. (g) The serum was collected and the protein concentration was quantified by using BCA assay kit. Every lane contains 30 μg total protein from three mice. Ponceau-S staining served as a loading control (n = 6). (h) Gene expression assay of adiponectin in epididymal adipose tissues (EAT) and inguinal adipose tissues (IAT) by qRT-PCR with the indicated probes (n = 6). All statistical analysis is based on one-way ANOVA. ^⁎P < 0.05, ^⁎⁎P < 0.01, ^⁎⁎⁎P < 0.001. Data are presented as mean ± SEM.