Skip to main content
. 2019 Jul 12;45:542–552. doi: 10.1016/j.ebiom.2019.07.009

Fig. 3.

Fig. 3

Lentiviral mediated RNAi knockdown of ANXA1 reduces the molecule's expression in AF-MSCs and their secretome. (a) Western blot analysis of ANXA1 in AF-MSCs, AF-MSC shscramble and AF-MSC shANXA1. Quantification was performed using the Quantity One software and the results were normalized to GAPDH positive control and then to AF-MSCs. (b) ELISA analysis of ANXA1 expression levels in shscramble-CM and shANXA1-CM. Data presented as mean ± SD of at least three independent experiments and analyzed by Student's test (****p < 0.0001). (c) Western blot analyses of the secreted ANXA1 in conditioned media (CM) derived from AF-MSC-shANXA1 and AF-MSC-shscramble. The densitometry analysis of the results was performed using the Quantity One software. Data presented as mean ± SD of two independent experiments and analyzed by Student's test (*p < 0.05).