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. 2019 Jul 2;116(29):14448–14455. doi: 10.1073/pnas.1814271116

Fig. 2.

Fig. 2.

Intracellular mechanical properties depend on extracellular mechanical cues. (A and B) Intracellular |G*| (mean ± SE) vs. frequency of nonmalignant MCF10A cells (A) and malignant MCF10-CA1 cells (B) adherent to 2D glass substrates (open circles), embedded in 3D lrECM (closed circles), and embedded in 3D HA (half-open circles). (C and D) Intracellular η (mean ± SE) vs. frequency of nonmalignant MCF10A cells (C) and malignant MCF10-CA1 cells (D) adherent to 2D glass substrates (open circles), embedded in 3D lrECM (closed circles), and embedded in 3D HA (half-open circles). Intersections with the horizontal dashed line at η = 1 of the vertical lines occur at crossover frequencies ωc. (E and F) Log2 ratios of |G*(ω)| in 3D ECM vs. on 2D substrates for nonmalignant MCF10A cells (E) and malignant MCF10-CA1 cells (F) (mean ± SD, averaged across frequency). (G) Log2 ratios of |G*(ω)| for malignant MCF10-CA1 cells vs. nonmalignant MCF10A cells. Power law coefficients (A) and exponents (B) and 95% confidence intervals are as follows: Nonmalignant cells in 2D: A = 11.74 (4.74, 18.74), b = 0.47 (0.40, 0.54); malignant cells in 2D: A = 1.14 (0.72, 1.55), b = 0.62 (0.58, 0.66); nonmalignant cells in 3D lrECM: A = 2.36 (0.91, 3.81), b = 0.49 (0.42, 0.56); malignant cells in 3D lrECM: A = 2.39 (1.21, 3.56), b = 0.62 (0.57, 0.68); nonmalignant cells in 3D HA: A = 2.26 (1.06, 3.46), b = 0.54 (0.48, 0.60); malignant cells in 3D HA: A = 6.36 (2.90, 9.81), b = 0.50 (0.44, 0.56). P values from 2-way ANOVA are shown above or below each bar in E–G (*P < 0.05 and **P < 0.01).