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. 2019 Jul 2;116(29):14573–14582. doi: 10.1073/pnas.1908547116

Fig. 2.

Fig. 2.

PARP-2 is required for PCa growth. (A) Relative growth rate of PCa cells transfected with two independent PARP-1 or PARP-2 siRNAs compared with nonspecific control (NC) siRNA (Upper). Gene knockdown (KD) efficiency was determined by Western blot (Lower). (B) Effect of PARP-1 or PARP-2 gene knockout (KO) on LNCaP cell proliferation using two independent sgRNAs (Upper). Gene KO was confirmed by Western blot (Lower). (C) Anchorage-independent growth of LNCaP cells after PARP-1 or PARP-2 KD. (D) Anchorage-independent growth of LNCaP cells after PARP-1 or PARP-2 KO. (E) Relative tumor volume of PARP-1 and PARP-2 KO LNCaP xenografts in vivo (10 mice per group). The GFP KO LNCaP cell line was used as a control. All data represent the mean ± SD. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons to the control group. *P < 0.05; **P < 0.01; ****P < 0.0001.