Figure 5. IL-4 and IFN-γ secreting CD4+ T cells in BALF and lung samples of infant and adult age primed mice after RSV challenge.
BALF and lung cells from infant (A) and adult (B) age primed mice (n=5) were collected at 5 days after challenge. CD4+ T cell epitope peptide F51−66 was used to stimulate BALF and lung cells in vitro, which were stained with phenotypic marker antibodies and cytokine antibodies, and then subsequently analyzed by Flowcytometry. The group labels are the same as in the Figure 2 legend. The results are presented as means with the SEM, and statistical significance was determined using one-way ANOVA with Tukey’s multiple comparison test performed in GraphPad Prism. ***, p < 0.0001, **p<0.005, * p<0.05.