Table 4.
Pheromone genes | Pollen Upregulated | Nectar Upregulated | Overlap Pollen | Overlap Nectar | |
---|---|---|---|---|---|
BP vs Control | 58 | 79 | 246 | 1 | 40* |
EBO vs Control | 152 | 79 | 246 | 0 | 71* |
Because foraging preference was a binary trait in the generalized linear model (i.e. either pollen or nectar), DEGs that were up-regulated in nectar foragers were by definition down-regulated in pollen foragers, and vice versa. Foraging-related DEGs were upregulated in pollen foragers (and downregulated in nectar foragers) when log fold change was greater than 0 and upregulated in nectar foragers (and downregulated in pollen foragers) when log fold change was less than zero. There was a significant overlap between pheromone-related genes and genes that were upregulated in nectar foragers
*significantly greater overlap of genes than expected by chance; P<0.001; hypergeometric test