Fig. 9.

The oncogenic hsa_circ_0058124/NOTCH3/GATAD2A axis in PTC cells. a Expression of Notch3 and GATAD2A in TPC-1 cells at protein level analyzed by western blot, after transfection with the indicated siRNAs. b, c The CCK-8 assays (b) and colony formation assays (c) were used to evaluate the cell growth after transfection with si-hsa_circ_0058124 or co-transfected with si-hsa_circ_0058124 and si-GATAD2A or si-NC in PTC cells. d Transwell assays were applied to evaluate the migration and invasion of PTC cells after transfection with si-hsa_circ_0058124 or co-transfected with si-hsa_circ_0058124 and si-GATAD2A or si-NC. e Immunohistochemistry analysis of GATAD2A protein levels in PTC tissues. Representative images were shown. Scale bar, 100 μm. f Representative images for the expression of GATAD2A in thyroid tumor tissues and normal thyroid tissues are shown with the fraction of samples with antibody staining/protein expressions evaluated as high, medium, low, or not detected based on the blue-scale color coding. Data are presented as the mean ± S.E.M., analyzed using independent samples student’s t-test. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001