Fig. 1.

Exo/R increased 5-FU resistance of RKO/P and HCT116 cells. a. Pictures of RKO/P and HCT116 cells treated with 30 μM and 10 μM 5-FU and exosomes after 72 h. b, c. RTCA of RKO/P (b) and HCT116 (c) cells treated with exosomes and exposed to 30 μM and 10 μM 5-FU. d, e. The absorbance showed the percentage of S-phase in RKO/P (d) and HCT116 (e) cells treated with 30 μM and 10 μM 5-FU and exosomes for 24 h. f. Representative apoptotic RKO/P and HCT116 cells treated with 30 μM and 10 μM 5-FU and exosomes for 72 h. Apoptotic cells were stained with Annexin V-APC and 7-AAD. g, h. Quantitative analysis of survival rate of RKO/P and HCT116 by FACS. *P < 0.05, **P < 0.01