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. 2019 Jul 19;38:320. doi: 10.1186/s13046-019-1314-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The identification of exosomes and uptake of exosomes by RKO/P cells. a. Representative images of exosomes derived from RKO/P and RKO/R taken by TEM (scale bar, 100 nm). b. Exosomal markers (CD63, CD9, ALIX, TSG101) and non-exosomal makers (LaminB1, TBP) were analyzed by WB in Exo/P, Exo/R, RKO/P and RKO/R. c. Histogram indicated diameter of exosomes purified from RKO/P and RKO/R analyzed by HSFCM. d. Confocal images of cells incubated with (+) or without (−) PKH-26 labelled Exo/P and Exo/R for 6 h. (scale bar, 10 μm)