Fig. 4.

Effects of miR-28-3p and miR-28-5p in cell apoptosis and death. The apoptosis of HONE-1 cells was examined by Annexin V-FITC/PI staining and analyzed by flow cytometry after transfection with mimics and inhibitors of miR-28-5p/-3p and negative control for 96 h (a). The early and late stages of apoptotic cells were all counted for cell apoptosis (b, c). Additionally, cell death was examined by trypan blue staining (d). The number of dying cells (trypan blue positive cells) were detected (e, f). The data are representative of three repeats. *P < 0.05, **P < 0.01, ***P < 0.001, vs negative control. Scale bar = 100 μm