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. 2019 Jul 19;12:65. doi: 10.1186/s13048-019-0541-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — In situ end labeling of DNA fragmentation on ovary sections. a Representative image of TUNEL immunostaining in granulosa cells of follicles. b Graphs show the mean ± SEM percentage of follicles with TUNEL-positive granulosa cells. * P < 0.05, **P < 0.01, vs. control- group; # P < 0.05, ## P < 0.01, vs. IR 2D- group. n = 10 mice/group. Arrow: TUNEL-positive granulosa cells, scale bars: 20 μm