Fig. 1.

Kinetics trace of hydrogen peroxide-dependent destruction of ascorbate by P. marinus lysate. a P. marinus osmotic lysate was added to assay buffer at 26 s. At 60 s, 0.1 mM hydrogen peroxide was added and ascorbate content was monitored at 290 nm. Both assay buffer and P. marinus lysate contained 0.4 mM ascorbate. b Proposed pathway for production and destruction of hydrogen peroxide in P. marinus, based on the presence of APX and lack of catalase activity