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. Author manuscript; available in PMC: 2019 Jul 20.
Published in final edited form as: J Am Chem Soc. 2019 Jan 8;141(2):758–762. doi: 10.1021/jacs.8b11521

Figure 2.

Figure 2

Flexizyme-enabled benchtop biosynthesis of a thiocillin scaffold. a) Sequence of the designer precursor peptide, including a pMCSG7-derived sequence, the LynD RS and TclM RS. The Trp-codon was reprogrammed to incorporate SecPh. EICs for SecPh and hexathiazole precursor peptide after LynD/TbtE treatment (b), hexathiazole and Dha-containing product after oxidative elimination (c), and fully cyclized thiocillin core (d).