Skip to main content
. 2019 Jul 12;25:5170–5180. doi: 10.12659/MSM.916815

Figure 5.

Figure 5

Knockdown of RASA2 suppressed medullary thyroid carcinoma (MTC) cell proliferation in vitro. (A) Knockdown efficiency of si-RASA2 in human MTC cell line TT was determined by quantitative real-time polymerase chain reaction (qRT-PCR). (B) Cell Counting Kit-8 analysis of TT cell viability was performed in si-NC or si-RASA2 treated TT cells. (C) Colony formation assay was carried out to evaluate the effects of si-RASA2 on cell proliferation of TT cells. (D) Relative expression of RASA2 was examined by qRT-PCR in immortalized normal thyroid follicular cell line NThy-ori-3.1, and 2 human MTC cell lines MZ-CRC-1 and TT. (E) Relative miR-31-3p expression was assessed in 22 pairs MTC and para-carcinoma tissues. (F) Correlation between the expression levels of RASA2 and miR-31-3p in MTC tissue samples (n=22). (G) Schematic diagram of the underlying molecular mechanism of miR-31-3p in the pathogenesis of MTC.