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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Andrology. 2019 Jan 20;7(5):603–609. doi: 10.1111/andr.12586

Figure 4. Epididymal amyloid matrix structure is altered in CRES KO mice.

Figure 4.

The epididymal amyloid matrix was isolated from the initial segment region from age-matched CRES wildtype (WT) and CRES knockout (KO) mice and incubated with the protein aggregation disease (PAD) reagent (Microsens Biotechnologies, London, UK) to pulldown amyloid structures (Whelly et al., 2012). Proteins were eluted from the PAD beads by incubation in Laemmli buffer at 65°C for 15 min, spotted on to formvar/carbon coated 200 mesh nickel grids (Ted Pella, Redding, CA, USA) and stained with 2% uranyl acetate. Images were captured with a Hitachi H-8100 transmission electron microscope. Bar, top panels, 500 nm; Bar, bottom panels, 100 nm.