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. Author manuscript; available in PMC: 2019 Jul 21.
Published in final edited form as: Sci Signal. 2018 Feb 20;11(518):eaao0422. doi: 10.1126/scisignal.aao0422

Fig. 7. Abl/Arg regulate cathepsin proform abundance via activation of NF-kB (p65/RelA).

Fig. 7.

(A,B) Cells transiently transfected with vector or constitutively active IKKβ-EE were transfected with the indicated siRNAs (72h; #1), serum-starved, and lysates blotted (A), or Gaussia luciferase secretion measured in the media from siRNA-transfected cells expressing cathepsin L or B promoter-luciferase constructs (B). Graphs are Mean±SEM, n=3. ***p<0.001; **p≤0.01; *p<0.05 using two-sample t-tests and Holm’s adjustment for multiple comparisons. (C) Cells transfected with scrambled or two independent p65 siRNAs, were treated with vehicle or nilotinib (16h), and lysates blotted. (D) Nilotinib treated cell lysates were blotted. pCrkL blots demonstrate efficiency of Abl/Arg inhibition by nilotinib.