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. 2019 Jul 7;2019:9151394. doi: 10.1155/2019/9151394

Figure 4.

Figure 4

rhIL-15 attenuated collagen synthesis and secretion induced by rhTGF-β1 stimulation in both RPTEC and HK-2 cells. (a) Immunofluorescent staining of collagen IV expression in RPTEC and HK-2 cells treated for 48h with rhTGF-β1 alone (3 ng/mL), rhIL-15 alone (1 ng/mL), or both cytokines. Original magnification ×63. Data is representative of three independent experiments. (b) The amount of collagen in 48h-treated HK-2 cell supernatants was quantify using the commercially Sirius Red collagen detection kit. Data are mean ±SEMs.