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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Acta Biomater. 2019 Mar 2;94:306–319. doi: 10.1016/j.actbio.2019.02.052

Figure 2. Primary rat cortical neural cells cultured on arabinofuranosylcytidine (AraC) -loaded silk films.

Figure 2.

(A) Fluorescence photographs of DIV10 cortical mixed cultures co-stained with glial cell marker, GFAP (green) and neuronal marker, β III tubulin (β3TB) (red). AraC at higher concentrations (indicated in numbers) reduced GFAP-positive cells. Toxic levels of AraC (>64 μM) induced neuronal cell death and breaking of neuronal connections. Scale bar, 100 μm. (B) Quantification of GFAP+ cell counts (per 100× microscopic field of view of 1.22 cm2) in DIV10 cultures on AraC in films (solid line), and with AraC in solution (dotted line). Error bar, standard error of mean (SEM), n = 3/group/time point. (C) Live cell percentages of cultures at 24hr with drug exposure (blue lines) and at DIV10 (red lines), on AraC in films (solid lines) and with AraC in solution (dotted lines). Error bar, SEM, n = 3/group/time point.