Figure 3.

RNA-sequencing analysis of liver transcriptomes in male wild type, Fxr^−/−, Tgr5^−/−, and DKO mice. A. Principal component analysis (PCA) of differentially expressed genes (DEGs) in liver of wild type, Fxr^−/−, Tgr5^−/− and DKO mice (n=6). Each dot represents data from individual mice. B. Venn diagram depicting overlapping and unique genes in Fxr^−/−, Tgr5^−/− and DKO mice vs. wild type mice. C. Selective representation of significantly up regulated pathways and diseases in DKO mice by pathway analysis and p-values. Detailed RNA-seq and analysis methods are described in Supplemental Materials.