Figure 7.

Effect of Western diet feeding on gene expression profile and bile acid metabolism in male wild type, Fxr^−/−, Tgr5^−/−, and DKO mice fed a high fat, high cholesterol Western diet for 16 weeks, n=5–8. A. Liver mRNA expression of genes involved in bile acid synthesis and regulation in chow-fed and Western diet-fed male mice. B. Ileum mRNA expression of genes involved in bile acid regulation. C. CYP7A1 protein expression in liver microsomes. D. CYP7A1 specific activity. E. Bile acid content in gallbladder (GB), intestine (Int) and liver, and total bile acid pool size. F. Serum (left) and fecal (right) bile acid content. WT-WD, wild type mice fed Western diet; Fxr-WD, Fxr^−/− mice fed Western diet; Tgr5-WD, Tgr5^−/− mice fed Western diet; DKO-WD, Fxr^−/−/Tgr5^−/− double knockout mice fed Western diet; * indicates p<0.05. An “*” indicates statistically significant difference (p<0.05) determined by one-way ANOVA (A-B, E-F) or Student’s t-test (C-D).