Figure 1. Schematic representation of vector constructions carrying TF and TFnt genes with major features labeled. A: The cloning/expression region of pET21a+/TF vector indicating the insertion of the TF gene consisting of N-terminal amidase-5 domain (D-glutamine-L-lysin endopeptidase activity) of LambdaSA2 prophage endolysin, a mid-protein amidase-2 domain of staphylococcal phage 2638A endolysin (amidase activity) and the mature S. simulans Lysostaphin (glycyl-glycine endopeptidase activity). pET21a+ carries an N-terminal T7-Tag sequence plus optional C-terminal His-Tag sequence. rbs—ribosomal binding site sequence; MCS—multiple cloning site. B: The cloning/expression region of pET26b+/TFnt vector indicating the insertion of the TFnt gene. pET26b+ carries an N-terminal pelB signal sequence for potential periplasmic localization of target gene. C: The cloning/expression region of pET28a+/TFnt vector indicating the insertion of the TFnt gene. D: The cloning/expression region of pEAQ-HT vector indicating the insertion of the TF and TFnt genes. 35S—Cauliflower mosaic virus 35S (35S) promoters; NosT—nopaline synthase transcriptional terminator; Nos—nopaline synthase promoter sequence; P19—gene coding the suppressor of gene silencing; NPTII—kanamycin resistance gene. E: The cloning/expression region of bipartite vector pGD5TGB1_L8823-MCS-CP3 indicating the insertion of the TF gene. TGB1 (L(88))—‘triple gene block 1’ with Leucine at the position 88 (Lim et al., 2010); gray boxes - duplicated CP (coat protein) subgenomic promoters.