Figure 3. Western blot analysis of TFnt produced in N. benthamiana on 6 dpi. The membrane was incubated with a 1:1000 dilution of polyclonal antibodies to Lysostaphin (AIC BIOTECH, Rockville MD, USA) followed by a 1:5000 dilution of goat anti-rabbit phosphatase-labeled antibodies (Kirkegaard & Perry Laboratories (KPL), Inc., Gaithersburg, MD) with subsequent membrane development by BCIP/NBT Membrane Phosphatase Substrate System (KPL). Lanes: M: Precision Plus Protein Kaleidoscope (Bio-Rad); 1: ‘control-mock’ (healthy plant); 2: ‘empty-pEAQ-HT’; 3: ‘pEAQ-HT/TFnt’; + - positive control (E. coli produced TFnt). The predicted TFnt MW = 72.9 kDa.
