Table 1. Oligonucleotide primers used for cloning.
| Primer | Nucleotide sequence [5′→3′]a | Restriction sites |
| 2438a146aaSalIF | ATGCATGCGTCGACGATGAAAAATCACAAGTATGTAG | SalI |
| 2638a400aaXhoIR | AGTCAGTCCTCGAGAATGCCATCTTTATTCTG | XhoI |
| LysostaphinSalIF | AGCTAGCTGTCGACGCTGCAACACATGAACATTCAG | SalI |
| pET21aStyIR | CGTTTAGAGGCCCCAAGGGGTTATG | StyI |
| AgeTFF | TAACACCGGTATGGAAATCAACACTGAAATAGCCATTGC | AgeI |
| XhoTFR | TAATCTCGAGTTATCAGTGATGGTGATGGTGATGCTCTAGCTTTATAGTTCC | XhoI |
| AgeTFnt1F | TAACACCGGTATGGAGATAAATACTGAGATCGCAATCG | AgeI |
| XhoTFntR | TAATCTCGAGTTATCAGTGATGGTGATGGTGATGTTTGATTGTAC | XhoI |
| NcoTFnt F | TAAGCCATGGAGATAAATACTGAGATCGCAATCGC | NcoI |
| TFMluIF3 | TAACACGCGTAACAATGGAAATCAACACTGAAATAGCCATTGC | MluI |
| TFSpeIR4 | TAATACTAGTTCATTAGTGATGGTGATGGTGATGCTCGAGCTTTATAGTTCCCCACAGAACACC | SpeI |
aRestriction sites are underlined; nucleotides in italics encode the 6xHis-tag.