Binding of each component of candidate dye FBBNA reveals that >50% of binding is attributable to the hydrophobic anchor region.
A, a total of 48 molecules of the high-retention factor component of FBBNA, pink in color, bound to each molecule of thyroglobulin. B, a total of 14 molecules of the low-retention factor component of FBBNA, orange in color, bound to each molecule of thyroglobulin. C, structures of FBBNA-PINK and FBBNA-ORANGE reveal that FBBNA-ORANGE has one fewer coupling agent than FBBNA-PINK. Error bars, S.D.