Figure 1.

Analysis of Cyp26a1 mRNA expression in the Cyp26a1^−/− mice compared with Cyp26a1^+/− and Cyp26a1^+/+ (control) mice. The Cyp26a1 deletion was induced either in adulthood (A) or in juvenile animals (B), and liver was collected 2 months after completion of tamoxifen treatment. A value of 0 was assigned for relative mRNA expression to mice in the Cyp26a1^−/− group that had no detectable Cyp26a1 mRNA in the liver. One female Cyp26a1^−/− mouse was excluded from all the analyses due to incomplete knockout of Cyp26a1 based on RT-PCR analysis. Each data point represents an individual mouse and is the average value of two RT-PCR analyses performed on separate days. Each analysis was performed in duplicate. The solid lines show the group geometric mean.