Figure 4.

Flow cytometry analysis of peripheral blood white blood cells (WBC). A shows total white blood cell counts in peripheral blood obtained from Cyp26a1^+/+ mice (circles) and Cyp26a1^−/− mice (squares) at different time points after tamoxifen injections. B–D show the flow cytometry analysis of peripheral blood in Cyp26a1^+/+ (control) and Cyp26a1^−/− mice 5 weeks after tamoxifen injections. The horizontal lines indicate mean values of the frequencies of CD3⁺ T cells (B), B220⁺ B cells (C), and myeloid cells (Gr-1⁺ cells) (D), and error bars indicate standard deviation (S.D.). Each data point represents an individual mouse. The filled and open symbols represent male and female mice, respectively. Significant differences were analyzed by unpaired Student's t test; *, p < 0.05. Representative flow cytometry plots are shown in Figs. S1 and S2.