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. 2019 Jul 15;11:6567–6579. doi: 10.2147/CMAR.S210637

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© 2019 Kuo et al.

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Negative effect of metformin on DEC1 expression in HeLa cells. HeLa cells were treated with (A) the indicated concentrations of metformin for 16 h; with (B) the indicated concentrations of metformin for 5 h plus 10 μM MG132 for 3 h; or with (C) 50 μg/mL CHX for indicated times. Cell lysates were subject to Western blot analysis using antibodies against AMPKα, p-AMPKα, ACC, p-ACC, FASN and DEC-1; ACTN served as the loading control. (D) HeLa cells were transiently transfected with the indicated amounts of pEGFP.DEC1 or 1 μg of empty pEGFP vector (control) and treated with 3 mM metformin for 20 h. Cell lysates were subjected to Western blot analysis using antibodies against DEC1, PARP and survivin; PCNA was the loading control. The results are representative of two independent experiments.