Figure 1.

Nucleic acid detection methodology. A: Synthesized or amplified fluorescently labeled DNA was added to the oligonucleotide microarray. B: Labeled DNA hybridizes to microarray probe sets each sharing sequences with the same flanking region and a central nucleotide triplet complementary to each of the reported codons at a drug resistance mutation (DRM) position. C: Multiplexed melt curve analysis was used to identify the most stable heterodimer and thereby detect the correct codon at the DRM position. RFU, relative fluorescence unit.