Figure 7.

Distribution of the mean change in melting temperatures (ΔT_ms) for each of the six probes in each of the 81 experiments (A) and receiver operating characteristic (ROC) curve analysis (B). A: The mean ΔT_m for a probe in an experiment was the mean values for a probe in each of the three probe sets. The distribution of these values was plotted according to the percentage of a codon in the experimental targets (100% for undiluted targets; 50%, 25%, and 10% for diluted AAC targets; and 50%, 75%, and 90% for AAA targets used to dilute AAC). Median ΔT_m for each codon dilution is shown. B: The ROC curves for targets at a concentration of 10%, 25%, 50%, and 100%. The sensitivity and specificity for detecting variant codons at these concentrations at optimized thresholds are shown.