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. 2019 Jul;21(4):580–592. doi: 10.1016/j.jmoldx.2019.02.005

Table 1.

Probe Sets Used to Distinguish between Six Codons at HIV-1 RT Codon 103

Probe set Codon 103 Probe sequence
1 AAA linker-5′-GTACTGTTACTGATTTTTTCTTTTTTAAACCTGC-blocker
1 AAG linker-5′-GTACTGTTACTGATTTCTTCTTTTTTAAACCTGC-blocker
1 AGA linker-5′-GTACTGTTACTGATTTTCTCTTTTTTAAACCTGC-blocker
1 AAC linker-5′-GTACTGTTACTGATTTGTTCTTTTTTAAACCTGC-blocker
1 AAT linker-5′-GTACTGTTACTGATTTATTCTTTTTTAAACCTGC-blocker
1 AGC linker-5′-GTACTGTTACTGATTTGCTCTTTTTTAAACCTGC-blocker
2 AAA linker-5′-GTACTGTTACTGATTTTTTCTTTTTTAACCCTGC-blocker
2 AAG linker-5′-GTACTGTTACTGATTTCTTCTTTTTTAACCCTGC-blocker
2 AGA linker-5′-GTACTGTTACTGATTTTCTCTTTTTTAACCCTGC-blocker
2 AAC linker-5′-GTACTGTTACTGATTTGTTCTTTTTTAACCCTGC-blocker
2 AAT linker-5′-GTACTGTTACTGATTTATTCTTTTTTAACCCTGC-blocker
2 AGC linker-5′-GTACTGTTACTGATTTGCTCTTTTTTAACCCTGC-blocker
3 AAA linker-5′-GTACTGTCATTGATTTTTTCTTTTTTAACCCTGC-blocker
3 AAG linker-5′-GTACTGTCATTGATTTCTTCTTTTTTAACCCTGC-blocker
3 AGA linker-5′-GTACTGTCATTGATTTTCTCTTTTTTAACCCTGC-blocker
3 AAC linker-5′-GTACTGTCATTGATTTGTTCTTTTTTAACCCTGC-blocker
3 AAT linker-5′-GTACTGTCATTGATTTATTCTTTTTTAACCCTGC-blocker
3 AGC linker-5′-GTACTGTCATTGATTTGCTCTTTTTTAACCCTGC-blocker

Probe set 1 was complementary to the most commonly occurring flanking sequence in the low- and middle-income countries sequence data set. Probe sets 2 and 3 differed from probe set 1 by one and three nucleotides, respectively, shown in bold. The central codon position was complementary to the six most commonly reported position 103 codons.